Description
Principle of the assay: </b<This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human immunoglobulin conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human anti-mitochondrial antibody M2 subtype (AMA-M2) bound in the initial step. The color development is stopped and the intensity of the color is measured